Fixatives and Solutions

Routine fixatives:

• 10% Neutral Buffered Formalin (NBF)
• 37 - 40% formalin………………………………………1000mL
• distilled water ……………………………………………9000mL
• sodium phosphate monobasic …………………40grams
• sodium phosphate dibasic (anhydrous)……65grams
  
  
• Bouin's
• Picric acid (saturated aqueous solution)………750mL
• 37 - 40% Formalin …………………………………………250mL
• Glacial acetic acid ……………………………………………50mL
  
  
• 4% Paraformaldehyde
• Paraformaldehyde ……………………40grams
• Deionized water…………………………500mL
• 10N Sodium Hydroxide………………20uL
• 10X PBS ………………………………………100mL
• Q.S. with deionized water …………400mL
  
  
• 70% Ethanol
• Ethanol ………………………………………11200mL
• Distilled water………………………………4800mL
  
  
• Carnoy 's
• 100% ETOH …………………………………600mL
• Chloroform……………………………………30mL
• Acetic acid ……………………………………100mL
  
  
• Davidson
• Glacial acetic acid ………………………………………100mL
• 95% Ethyl alcohol…………………………………………300mL
• 10% Neutral Buffered Formalin (NBF)…………200mL
• Distilled water………………………………………………300mL
  
  
• Zinc
• Distilled water………………………………………2000mL
• Formalin ………………………………………………50mL
• Zinc chloride…………………………………………15grams
  
  
• Telley's
• 100% ETOH ……………………………………700mL
• Formaldehyde…………………………………50mL
• Glacial acetic acid……………………………50mL
• Distilled water…………………………………200mL

Other Solutions

• Formic Acid Sodium Citrate Decal
• Distilled water……………………………………1500mL
• Sodium citrate, dehydrate…………………200mL
• Formic acid …………………………………………500mL

 

• EDTA Decal
  • Distilled water…………………………………3000mL
  • EDTA…………………………………………………200grams
  • Sucrose ……………………………………………280grams