Karyotyping

Karyotyping (Human)
• Standard analysis of metaphase chomosomes using G-banding technique.
• Monitoring of established cell lines using classical and/or molecular cytogenetic techniques to rule out interspecies culture contamination.
• Characterize tumor related cell lines for chromosome rearrangement.
• Special studies using a variety of banding techniques including G, C, and R.

Karyotyping (Mammalian)
• Standard analysis of metaphase chromosomes from a wide variety of species including but not restricted to mouse.

Testing Options:

Identifies chromosomal aberrations
Translocations (balanced)	Yes
Translocations (unbalanced)	Yes
Copy number changes	Yes
Additions (>10MB)	Yes
Deletions (>10MB)	Yes
Deletions of specific genes/loci	No
Inversions	Yes
Insertions	Sometimes
Identifies presence of markers	Yes
Distinguishes between double minutes and homogeneously staining regions	Yes
Identifies chromosomal origins	Sometimes
Identifies specific p/q arms/bands	Yes
Requires specifically labeled probes	No
More than two probes at one time
Requires viable material	Yes
Requires metaphase spreads	Yes
Applicable to interphase nuclei	No
Applicable to DNA extracted from paraffin embedded tissue	No
Affected by normal tissue contamination	Yes
Identifies tumor heterogeneity	Yes
Applicable for studies in other species	Yes

Sample Requirements:
Peripheral blood: 5-10 mls in Heparin container
Culture: Culture must be actively growing with media supplied. A T-75 flask is preferred. Tissue culture flask must not be vented. This prevents spillage and contamination during transit. Do not fill beyond recommendation of flask i.e. (to the absolute top of flask or tube).

Please inquire if your sample varies from the listed recommendations.  Please inquire for likely turnaround time.