• Mapping cancer related genes, identify marker chromosomes, the origin of which is unidentifiable by classical cytogenetics methods, estimation of
gene copy number in interphase.
• Telomere and pan centromeric probes mouse and/or human.
• Localization of BAC clones.
| Identifies chromosomal aberrations |
|
| Translocations (balanced) |
Yes |
| Translocations (unbalanced) |
Yes |
| Copy number changes |
Yes |
| Additions (>10MB) |
Yes |
| Deletions (>10MB) |
Yes |
| Deletions of specific genes/loci |
Yes |
| Inversions |
Yes |
| Insertions |
Yes |
| Identifies presence of markers |
Yes |
| Distinguishes between double minutes and homogeneously staining regions |
Yes |
| Identifies chromosomal origins |
Yes |
| Identifies specific p/q arms/bands |
Yes |
| Requires specifically labeled probes |
Yes |
| More than two probes at one time |
Sometimes |
| Requires viable material |
Sometimes |
| Requires metaphase spreads |
Yes |
| Applicable to interphase nuclei |
Yes |
| Applicable to DNA extracted from paraffin embedded tissue |
Yes |
| Affected by normal tissue contamination |
Yes |
| Identifies tumor heterogeneity |
Yes |
| Applicable for studies in other species |
Yes |
