• Assessment of chromosome loss, gain or amplification in a sample of genomic DNA by competitive hybridization of two genomes against a target cell.
| Identifies chromosomal aberrations |
|
| Translocations (balanced) |
No |
| Translocations (unbalanced) |
Yes |
| Copy number changes |
Yes |
| Additions (>10MB) |
Yes |
| Deletions (>10MB) |
Yes |
| Deletions of specific genes/loci |
Sometimes |
| Inversions |
No |
| Insertions |
No |
| Identifies presence of markers |
Sometimes |
| Distinguishes between double minutes and homogeneously staining regions |
No |
| Identifies chromosomal origins |
Sometimes |
| Identifies specific p/q arms/bands |
Sometimes |
| Requires specifically labeled probes |
Yes |
| More than two probes at one time |
Yes |
| Requires viable material |
No |
| Requires metaphase spreads |
Yes |
| Applicable to interphase nuclei |
No |
| Applicable to DNA extracted from paraffin embedded tissue |
Yes |
| Affected by normal tissue contamination |
Yes |
| Identifies tumor heterogeneity |
No |
| Applicable for studies in other species |
Yes |
